-----------------------
Start of variable list:
-----------------------
Sample_ID:
LZ_M19
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
STRANDEDNESS_featureCount:
2
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion_rerun/Scripts/07b_Extract_Counts_featureCounts
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Sun May 13 15:13:35 EDT 2018
Running on node : scc-kb5
Current directory : /restricted/projectnb/waxmanlab/kkarri/liver_zonation/infusion_rerun/Scripts/07b_Extract_Counts_featureCounts
Current job ID : 6184686
Current job name : Step_07b_LZ_M19
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/6184686.1.linga

Loading required modules...


List files in scratch directory:

total 366M
drwxr-xr-x   2 kkarri waxmanlab 4.0K May 13 15:13 .
drwxrwxrwt. 50 root   root      268K May 13 15:13 ..
-rw-r--r--   1 kkarri waxmanlab    0 May 13 15:13 LZ_M19_featureCounts.out
-rw-r--r--   1 kkarri waxmanlab 295M May 13 15:13 LZ_M19_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  72M May 13 15:13 RefSeq_GeneBody.gtf

Starting to run my commands


Starting featureCounts

GTF file name: RefSeq_GeneBody
Rscript Parse_GTF.R RefSeq_GeneBody.gtf GeneSym_List.txt
[1] "Print arguments:"
[1] "-----------------"
[1] "GTF_File:"
[1] "RefSeq_GeneBody.gtf"
[1] "Subset_List:"
[1] "GeneSym_List.txt"
[1] "-----------------"
[1] "Loading RefSeq_GeneBody.gtf"
[1] "Loading GeneSym_List.txt"
[1] "Number of gene symbols in common:"
[1] "1713"
[1] "Percentage overlap:"
[1] "98%"
[1] "How many GeneSym in the GTF list and not in Subset list:"
[1] "(Expect high number)"
[1] 22484
[1] "How many GeneSym in the Subset list and not in GTF list:"
[1] "(Due to updated RefSeq gene annotations)"
[1] 27
[1] "Check out GeneSym_assign_all_features.gtf!"
[1] "GeneSym_assign_only1_feature.gtf!"
Running assign_all_features counting
featureCounts -O -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_all_features.gtf -o LZ_M19_assign_all_features.out LZ_M19'_sorted'.bam
Running assign_only1_feature counting
featureCounts -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_only1_feature.gtf -o LZ_M19_assign_only1_feature.out LZ_M19'_sorted'.bam
Processing LZ_M19_assign_all_features.out
Done processing file.
Processing LZ_M19_assign_only1_feature.out
Done processing file.

Ending featureCounts


List files in scratch

total 926M
drwxr-xr-x   2 kkarri waxmanlab 4.0K May 13 15:18 .
drwxrwxrwt. 49 root   root      268K May 13 15:18 ..
-rw-r--r--   1 kkarri waxmanlab  14K May 13 15:18 GeneSym_List.txt
-rw-r--r--   1 kkarri waxmanlab 1.3M May 13 15:18 GeneSym_assign_all_features.gtf
-rw-r--r--   1 kkarri waxmanlab  67M May 13 15:18 GeneSym_assign_only1_feature.gtf
-rw-r--r--   1 kkarri waxmanlab  17K May 13 15:18 LZ_M19_assign_all_features.out
-rw-r--r--   1 kkarri waxmanlab  290 May 13 15:18 LZ_M19_assign_all_features.out.summary
-rw-r--r--   1 kkarri waxmanlab 224K May 13 15:18 LZ_M19_assign_only1_feature.out
-rw-r--r--   1 kkarri waxmanlab  296 May 13 15:18 LZ_M19_assign_only1_feature.out.summary
-rw-r--r--   1 kkarri waxmanlab 241K May 13 15:18 LZ_M19_featureCounts.out
-rw-r--r--   1 kkarri waxmanlab  649 May 13 15:18 LZ_M19_featureCounts.out.summary
-rw-r--r--   1 kkarri waxmanlab 295M May 13 15:13 LZ_M19_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 492M May 13 15:17 LZ_M19_sorted.bam
-rwxr-xr-x   1 kkarri waxmanlab 6.8K May 13 15:18 Parse_GTF.R
-rw-r--r--   1 kkarri waxmanlab  72M May 13 15:13 RefSeq_GeneBody.gtf
==========================================================
Finished on : Sun May 13 15:18:45 EDT 2018
0 hours, 5 minutes and 10 seconds elapsed.
==========================================================