========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P B3-MAA100138-3_56_F-1-1_sorted.bam || || || || Output file : B3-MAA100138-3_56_F-1-1_featureCounts.out || || Annotations : ncRNA_genebodies_for_counting.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file ncRNA_genebodies_for_counting.gtf ... || || Features : 37893 || || Meta-features : 37893 || || Chromosomes/contigs : 32 || || || || Process BAM file B3-MAA100138-3_56_F-1-1_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR6575753.161 145 chr16 90225406 50 100M = 90221140 || || -4366 N # NH:i:1 || || SRR6575753.162 83 chrM 4332 50 100M = 4161 -271 N || || # NH:i:1 || || 121077 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 732391 || || Successfully assigned fragments : 514658 (70.3%) || || Running time : 0.48 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//