Warning message: In rbind(c("gene_id Xkr4", " transcript_id NM_001011874", " gene_name Xkr4" : number of columns of result is not a multiple of vector length (arg 665745) ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P F10-MAA000377-3_9_M-1-1_sorted.bam || || || || Output file : F10-MAA000377-3_9_M-1-1_assign_all_features.out || || Annotations : GeneSym_assign_all_features.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_all_features.gtf ... || || Features : 6105 || || Meta-features : 1713 || || Chromosomes/contigs : 25 || || || || Process BAM file F10-MAA000377-3_9_M-1-1_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR6575592.161 73 chr17 39983332 50 100M * 0 0 N || || # NH:i:1 || || SRR6575592.163 83 chrX 34338453 50 100M = 34337868 || || -685 N # NH:i:1 || || 101637 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 534809 || || Successfully assigned fragments : 5990 (1.1%) || || Running time : 0.45 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================// ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P F10-MAA000377-3_9_M-1-1_sorted.bam || || || || Output file : F10-MAA000377-3_9_M-1-1_assign_only1_feature ... || || Annotations : GeneSym_assign_only1_feature.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_only1_feature.gtf ... || || Features : 315310 || || Meta-features : 22576 || || Chromosomes/contigs : 124 || || || || Process BAM file F10-MAA000377-3_9_M-1-1_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR6575592.4 89 chr4 107579934 50 100M * 0 0 N || || # NH:i:1 || || SRR6575592.5 81 chr4 99637318 50 100M = 99634170 -3 || || 248 N # NH:i:1 || || 101637 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 534809 || || Successfully assigned fragments : 523711 (97.9%) || || Running time : 0.42 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//