========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P H3-MAA100140-3_57_F-1-1_sorted.bam || || || || Output file : H3-MAA100140-3_57_F-1-1_featureCounts.out || || Annotations : genes.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file genes.gtf ... || || Features : 210274 || || Meta-features : 17421 || || Chromosomes/contigs : 118 || || || || Process BAM file H3-MAA100140-3_57_F-1-1_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR6576126.5 153 chr2 31336622 50 62M5325N38M * 0 0 || || N # NH:i:1 || || SRR6576126.7 97 chr7 52713507 50 100M = 52715038 16 || || 31 N # NH:i:1 || || 235686 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 942282 || || Successfully assigned fragments : 722643 (76.7%) || || Running time : 0.59 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//