cp: cannot stat `/restricted/projectnb/waxmanlab/kkarri/tabula_muris/GTF_Files/intronic_only_gene_models_ncRNA_for_counting.gtf': No such file or directory Error: Unable to open file Intron_Only_Regions.gtf. Exiting. [bam_header_read] EOF marker is absent. The input is probably truncated. [bam_header_read] invalid BAM binary header (this is not a BAM file). /var/spool/sge/scc-pg6/job_scripts/8814045: line 315: 109559 Segmentation fault samtools sort -n ${INPUT_BAM} ${Sample_ID}'_sorted' ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 unknown file || || ? O2-MAA000907-3_11_M-1-1_sorted.bam || || || || Output file : O2-MAA000907-3_11_M-1-1_featureCounts.out || || Annotations : exonic_only_gene_models_ncRNA_for_counting.g ... || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file exonic_only_gene_models_ncRNA_for_counting.gtf ... || || Features : 210548 || || Meta-features : 37893 || || Chromosomes/contigs : 32 || || || || Process Unknown file O2-MAA000907-3_11_M-1-1_sorted.bam... || || Single-end reads are included. || || Failed to open file O2-MAA000907-3_11_M-1-1_sorted.bam || || No counts were generated for this file. || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//