========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P P21-MAA000907-3_11_M-1-1_sorted.bam || || || || Output file : P21-MAA000907-3_11_M-1-1_featureCounts.out || || Annotations : ncRNA_exon_for_counting.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : no || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file ncRNA_exon_for_counting.gtf ... || || Features : 2365767 || || Meta-features : 37893 || || Chromosomes/contigs : 32 || || || || Process BAM file P21-MAA000907-3_11_M-1-1_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR6576781.178 147 chr19 5423846 50 46M290N54M = 5423 || || 781 -455 N # NH:i:1 || || SRR6576781.179 73 chr11 115363338 50 79M929N21M * 0 || || 0 N # NH:i:1 || || 113860 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 424592 || || Successfully assigned fragments : 333170 (78.5%) || || Running time : 0.44 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//