----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: G123_M13 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_New Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Fri Dec 7 19:06:11 EST 2018 Running on node : scc-tm3 Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/08a_Extract_Counts_HTSeq Current job ID : 27240 Current job name : Step_08a_G123_M13 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/27240.1.linga Loading required modules... List files in scratch directory: total 1.5G drwx------ 2 kkarri waxmanlab 4.0K Dec 7 19:06 . drwxrwxrwt. 21 root root 36K Dec 7 19:06 .. -rw-r--r-- 1 kkarri waxmanlab 0 Dec 7 19:06 G123_M13_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.4G Dec 7 19:06 G123_M13_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 72M Dec 7 19:06 RefSeq_GeneBody.gtf Starting to run my commands Starting samtools and htseq-count samtools view G123_M13'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf > G123_M13_HTSeq.out Ending samtools and htseq-count List files in scratch total 3.4G drwx------ 2 kkarri waxmanlab 4.0K Dec 7 19:19 . drwxrwxrwt. 17 root root 36K Dec 7 19:46 .. -rw-r--r-- 1 kkarri waxmanlab 245K Dec 7 19:46 G123_M13_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.4G Dec 7 19:06 G123_M13_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 2.0G Dec 7 19:19 G123_M13_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 72M Dec 7 19:06 RefSeq_GeneBody.gtf ========================================================== Finished on : Fri Dec 7 19:46:50 EST 2018 0 hours, 40 minutes and 39 seconds elapsed. ==========================================================