-----------------------
Need shift 1 command (have more than 9 arguments):
-----------------------
-----------------------
Start of variable list:
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Sample_ID:
G123_M4
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_New
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
reverse
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/08a_Extract_Counts_HTSeq
ANNOTATION_FILE:
Intron_Only_Regions.gtf
FEATURE_TYPE:
Intronic_Only
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Fri Dec  7 19:06:10 EST 2018
Running on node : scc-tm4
Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_New/Scripts/08a_Extract_Counts_HTSeq
Current job ID : 27203
Current job name : Step_08a_G123_M4
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/27203.1.linga

Loading required modules...


List files in scratch directory:

total 1.9G
drwx------   2 kkarri waxmanlab 4.0K Dec  7 19:06 .
drwxrwxrwt. 15 root   root       20K Dec  7 19:06 ..
-rw-r--r--   1 kkarri waxmanlab    0 Dec  7 19:06 G123_M4_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 1.8G Dec  7 19:06 G123_M4_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  18M Dec  7 19:06 Intron_Only_Regions.gtf

Starting to run my commands


Starting samtools and htseq-count


Starting splice read filter


Ending splice read filter

samtools view G123_M4'_sorted'.bam | htseq-count --stranded=reverse --type=Intronic_Only --idattr=gene_id --order=name --mode=intersection-nonempty - Intron_Only_Regions.gtf  > G123_M4_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 38G
drwx------   2 kkarri waxmanlab 4.0K Dec  7 20:00 .
drwxrwxrwt. 15 root   root       20K Dec  7 19:06 ..
-rw-r--r--   1 kkarri waxmanlab 243K Dec  7 20:00 G123_M4_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 1.7G Dec  7 19:16 G123_M4_primary_no_splice.bam
-rw-r--r--   1 kkarri waxmanlab 1.8G Dec  7 19:06 G123_M4_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 2.3G Dec  7 19:30 G123_M4_sorted.bam
-rw-r--r--   1 kkarri waxmanlab   99 Dec  7 20:00 HTSeq_Extra_Lines.txt
-rw-r--r--   1 kkarri waxmanlab  922 Dec  7 19:07 Header.txt
-rw-r--r--   1 kkarri waxmanlab  18M Dec  7 19:06 Intron_Only_Regions.gtf
-rwxr-xr-x   1 kkarri waxmanlab  38K Dec  7 20:00 RefSeq_Genes_Zero_Count.txt
-rw-r--r--   1 kkarri waxmanlab  12G Dec  7 19:10 temp1.sam
-rw-r--r--   1 kkarri waxmanlab  11G Dec  7 19:10 temp2.sam
-rw-r--r--   1 kkarri waxmanlab  11G Dec  7 19:11 temp3.sam
==========================================================
Finished on : Fri Dec  7 20:00:37 EST 2018
0 hours, 54 minutes and 27 seconds elapsed.
==========================================================