----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: G168_G169_M5 Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G168_G169/G168/TCPOBOP_frStrand_Env/TCPOBOP Sample_Labels_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G168_G169/G168/TCPOBOP_frStrand_Env/TCPOBOP/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G168_G169/G168/TCPOBOP_frStrand_Env/TCPOBOP/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: Intron_Only_Regions.gtf FEATURE_TYPE: Intronic_Only ----------------------- End of variable list ----------------------- ========================================================== Starting on : Mon Apr 15 21:35:25 EDT 2019 Running on node : scc-wh2 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G168_G169/G168/TCPOBOP_frStrand_Env/TCPOBOP/Scripts/08a_Extract_Counts_HTSeq Current job ID : 5225979 Current job name : Step_08a_G168_G169_M5 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/5225979.1.w Loading required modules... List files in scratch directory: total 1.9G drwx------ 2 kkarri waxmanlab 4.0K Apr 15 21:47 . drwxrwxrwt. 21 root root 72K Apr 15 21:46 .. -rw-r--r-- 1 kkarri waxmanlab 0 Apr 15 21:47 G168_G169_M5_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.9G Apr 15 21:45 G168_G169_M5_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 18M Apr 15 21:45 Intron_Only_Regions.gtf Starting to run my commands Starting samtools and htseq-count Starting splice read filter Ending splice read filter samtools view G168_G169_M5'_sorted'.bam | htseq-count --stranded=reverse --type=Intronic_Only --idattr=gene_id --order=name --mode=intersection-nonempty - Intron_Only_Regions.gtf > G168_G169_M5_HTSeq.out Ending samtools and htseq-count List files in scratch total 39G drwx------ 2 kkarri waxmanlab 4.0K Apr 15 22:18 . drwxrwxrwt. 19 root root 72K Apr 15 22:16 .. -rw-r--r-- 1 kkarri waxmanlab 233K Apr 15 22:18 G168_G169_M5_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1013M Apr 15 21:55 G168_G169_M5_primary_no_splice.bam -rw-r--r-- 1 kkarri waxmanlab 1.9G Apr 15 21:45 G168_G169_M5_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 1.9G Apr 15 22:04 G168_G169_M5_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 99 Apr 15 22:18 HTSeq_Extra_Lines.txt -rw-r--r-- 1 kkarri waxmanlab 890 Apr 15 21:49 Header.txt -rw-r--r-- 1 kkarri waxmanlab 18M Apr 15 21:45 Intron_Only_Regions.gtf -rwxr-xr-x 1 kkarri waxmanlab 38K Apr 15 22:18 RefSeq_Genes_Zero_Count.txt -rw-r--r-- 1 kkarri waxmanlab 17G Apr 15 21:51 temp1.sam -rw-r--r-- 1 kkarri waxmanlab 8.7G Apr 15 21:51 temp2.sam -rw-r--r-- 1 kkarri waxmanlab 8.7G Apr 15 21:52 temp3.sam ========================================================== Finished on : Mon Apr 15 22:18:12 EDT 2019 0 hours, 42 minutes and 47 seconds elapsed. ==========================================================