[samopen] SAM header is present: 22 sequences.
[bam_sort_core] merging from 13 files...

        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
	v1.4.6-p5

//========================== featureCounts setting ===========================\\
||                                                                            ||
||             Input files : 1 BAM file                                       ||
||                           P G168_G169_M18_sorted.bam                       ||
||                                                                            ||
||             Output file : G168_G169_M18_assign_all_features.out            ||
||             Annotations : GeneSym_assign_all_features.gtf (GTF)            ||
||                                                                            ||
||                 Threads : 16                                               ||
||                   Level : meta-feature level                               ||
||              Paired-end : yes                                              ||
||         Strand specific : inversed                                         ||
||      Multimapping reads : not counted                                      ||
|| Multi-overlapping reads : counted                                          ||
||       Read orientations : fr                                               ||
||                                                                            ||
||          Chimeric reads : counted                                          ||
||        Both ends mapped : not required                                     ||
||                                                                            ||
\\===================== http://subread.sourceforge.net/ ======================//

//================================= Running ==================================\\
||                                                                            ||
|| Load annotation file GeneSym_assign_all_features.gtf ...                   ||
||    Features : 3913                                                         ||
||    Meta-features : 524                                                     ||
||    Chromosomes/contigs : 25                                                ||
||                                                                            ||
|| Process BAM file G168_G169_M18_sorted.bam...                               ||
||    Paired-end reads are included.                                          ||
||    Assign fragments (read pairs) to features...                            ||
||    Found reads that are not properly paired.                               ||
||    (missing mate or the mate is not the next read)                         ||
||    Below are the two reads that are not properly paired:                   ||
||       K00124:51:HT7MFBBXX:2:1101:1387:37401   73   chr1   55134969   50    ||
||        150M   *   0   0   N   #   NH:i:1                                   ||
||       K00124:51:HT7MFBBXX:2:1101:1387:42042   89   chrM   4565   50   150  ||
||       M   *   0   0   N   #   NH:i:1                                       ||
||    2722083 reads have missing mates.                                       ||
||    Input was converted to a format accepted by featureCounts.              ||
||    Total fragments : 10012292                                              ||
||    Successfully assigned fragments : 21812 (0.2%)                          ||
||    Running time : 4.55 minutes                                             ||
||                                                                            ||
||                         Read assignment finished.                          ||
||                                                                            ||
\\===================== http://subread.sourceforge.net/ ======================//


        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
	v1.4.6-p5

//========================== featureCounts setting ===========================\\
||                                                                            ||
||             Input files : 1 BAM file                                       ||
||                           P G168_G169_M18_sorted.bam                       ||
||                                                                            ||
||             Output file : G168_G169_M18_assign_only1_feature.out           ||
||             Annotations : GeneSym_assign_only1_feature.gtf (GTF)           ||
||                                                                            ||
||                 Threads : 16                                               ||
||                   Level : meta-feature level                               ||
||              Paired-end : yes                                              ||
||         Strand specific : inversed                                         ||
||      Multimapping reads : not counted                                      ||
|| Multi-overlapping reads : not counted                                      ||
||       Read orientations : fr                                               ||
||                                                                            ||
||          Chimeric reads : counted                                          ||
||        Both ends mapped : not required                                     ||
||                                                                            ||
\\===================== http://subread.sourceforge.net/ ======================//

//================================= Running ==================================\\
||                                                                            ||
|| Load annotation file GeneSym_assign_only1_feature.gtf ...                  ||
||    Features : 189176                                                       ||
||    Meta-features : 19957                                                   ||
||    Chromosomes/contigs : 32                                                ||
||                                                                            ||
|| Process BAM file G168_G169_M18_sorted.bam...                               ||
||    Paired-end reads are included.                                          ||
||    Assign fragments (read pairs) to features...                            ||
||    Found reads that are not properly paired.                               ||
||    (missing mate or the mate is not the next read)                         ||
||    Below are the two reads that are not properly paired:                   ||
||       K00124:51:HT7MFBBXX:2:1101:1387:37401   73   chr1   55134969   50    ||
||        150M   *   0   0   N   #   NH:i:1                                   ||
||       K00124:51:HT7MFBBXX:2:1101:1387:42042   89   chrM   4565   50   150  ||
||       M   *   0   0   N   #   NH:i:1                                       ||
||    2722083 reads have missing mates.                                       ||
||    Input was converted to a format accepted by featureCounts.              ||
||    Total fragments : 10012292                                              ||
||    Successfully assigned fragments : 1323703 (13.2%)                       ||
||    Running time : 4.29 minutes                                             ||
||                                                                            ||
||                         Read assignment finished.                          ||
||                                                                            ||
\\===================== http://subread.sourceforge.net/ ======================//