[bam_sort_core] merging from 12 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P G168_G169_M20_sorted.bam || || || || Output file : G168_G169_M20_assign_all_features.out || || Annotations : GeneSym_assign_all_features.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_all_features.gtf ... || || Features : 5739 || || Meta-features : 1725 || || Chromosomes/contigs : 25 || || || || Process BAM file G168_G169_M20_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || K00124:51:HT7MFBBXX:2:1101:1458:34606 99 chrX 71024350 50 || || 150M = 71024367 461 N # NH:i:1 || || K00124:51:HT7MFBBXX:2:1101:1458:35520 83 chr16 23081588 50 || || 150M = 23079113 -2625 N # NH:i:1 || || 2818894 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 9398063 || || Successfully assigned fragments : 26940 (0.3%) || || Running time : 4.09 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================// ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P G168_G169_M20_sorted.bam || || || || Output file : G168_G169_M20_assign_only1_feature.out || || Annotations : GeneSym_assign_only1_feature.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_only1_feature.gtf ... || || Features : 206368 || || Meta-features : 22472 || || Chromosomes/contigs : 32 || || || || Process BAM file G168_G169_M20_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || K00124:51:HT7MFBBXX:2:1101:1387:33217 73 chr11 8332511 50 || || 150M * 0 0 N # NH:i:1 || || K00124:51:HT7MFBBXX:2:1101:1387:36205 73 chr19 60853872 50 || || 150M * 0 0 N # NH:i:1 || || 2818894 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 9398063 || || Successfully assigned fragments : 7849779 (83.5%) || || Running time : 4.41 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//