##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543821_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26776805
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43334393330347	32.0	32.0	32.0	27.0	32.0
2	30.763139963860514	32.0	32.0	32.0	32.0	32.0
3	33.149981112384395	37.0	32.0	37.0	27.0	37.0
4	34.70178256890618	37.0	37.0	37.0	27.0	37.0
5	35.380560040677	37.0	37.0	37.0	32.0	37.0
6	38.627278011697065	41.0	41.0	41.0	32.0	41.0
7	38.38747288931596	41.0	41.0	41.0	32.0	41.0
8	38.435375691760086	41.0	41.0	41.0	32.0	41.0
9	38.55857179375956	41.0	41.0	41.0	32.0	41.0
10-11	38.646070526338	41.0	41.0	41.0	32.0	41.0
12-13	38.66504956435243	41.0	41.0	41.0	32.0	41.0
14-15	38.636230480074076	41.0	41.0	41.0	32.0	41.0
16-17	38.51281243225247	41.0	41.0	41.0	32.0	41.0
18-19	38.571261321132226	41.0	41.0	41.0	32.0	41.0
20-21	38.62837244772108	41.0	41.0	41.0	32.0	41.0
22-23	38.52101871377111	41.0	41.0	41.0	32.0	41.0
24-25	38.53166819192954	41.0	41.0	41.0	32.0	41.0
26-27	38.65980889056779	41.0	41.0	41.0	32.0	41.0
28-29	38.512131992595826	41.0	41.0	41.0	32.0	41.0
30-31	38.55669791448233	41.0	41.0	41.0	32.0	41.0
32-33	38.7137366089793	41.0	41.0	41.0	34.5	41.0
34-35	38.66983486267313	41.0	41.0	41.0	34.5	41.0
36-37	38.52475698061224	41.0	41.0	41.0	32.0	41.0
38-39	38.469503810116244	41.0	41.0	41.0	32.0	41.0
40-41	38.491885479980226	41.0	41.0	41.0	32.0	41.0
42-43	38.451738522970714	41.0	41.0	41.0	32.0	41.0
44-45	38.401151566310716	41.0	39.0	41.0	32.0	41.0
46-47	38.405994816368846	41.0	41.0	41.0	32.0	41.0
48-49	38.369279000079715	41.0	37.0	41.0	32.0	41.0
50-51	38.306771857801735	41.0	37.0	41.0	32.0	41.0
52-53	38.33859440707762	41.0	37.0	41.0	32.0	41.0
54-55	38.33689136636332	41.0	37.0	41.0	32.0	41.0
56-57	38.32432116665218	41.0	37.0	41.0	32.0	41.0
58-59	38.3379610284117	41.0	37.0	41.0	32.0	41.0
60-61	38.29327848835789	41.0	37.0	41.0	32.0	41.0
62-63	38.3238179934665	41.0	37.0	41.0	32.0	41.0
64-65	38.31890603430924	41.0	37.0	41.0	32.0	41.0
66-67	38.23903510704379	41.0	37.0	41.0	32.0	41.0
68-69	38.246662049443955	41.0	37.0	41.0	32.0	41.0
70-71	38.07273561250212	41.0	37.0	41.0	32.0	41.0
72-73	38.18443029755016	41.0	37.0	41.0	32.0	41.0
74-75	38.21025650354828	41.0	37.0	41.0	32.0	41.0
76	37.27421787648464	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20001.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	30.0
13	207.0
14	1070.0
15	4182.0
16	12592.0
17	28147.0
18	45666.0
19	62771.0
20	75902.0
21	87151.0
22	99061.0
23	112042.0
24	126888.0
25	144070.0
26	162602.0
27	186491.0
28	213745.0
29	246262.0
30	286793.0
31	335593.0
32	395477.0
33	473026.0
34	582382.0
35	741671.0
36	990716.0
37	1444314.0
38	2522611.0
39	6462172.0
40	1.0913169E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.65565816982965	19.347506173646224	14.204222825444104	30.792612831080024
2	22.33291158038196	27.56830930758664	28.63243960829071	21.466339503740688
3	21.490473974395446	28.66869301729758	27.682764354068595	22.158068654238374
4	25.379026662498212	32.85342493476818	19.400455851934268	22.36709255079935
5	24.65698847417886	35.0117195713665	20.06387478473132	20.267417169723327
6	20.067534713852016	37.106004833945704	20.858602790114293	21.967857662087987
7	20.178836428547108	21.55240804550898	33.79344666826203	24.47530885768188
8	21.178894602101593	23.75716195758293	26.22955145296237	28.834391987353108
9	22.048287990477576	23.533916489991224	27.59242730045031	26.825368219080886
10-11	23.8542547894918	30.863946109984465	21.27893408462045	24.00286501590329
12-13	23.61054373135203	25.174828180403907	25.140229460801333	26.074398627442726
14-15	22.738319679318725	28.042344821021292	25.66271302803278	23.556622471627207
16-17	23.110606860580372	28.083603930717356	25.00173508974871	23.804054118953566
18-19	22.832406549834	28.284648405625042	25.26161546580733	23.62132957873363
20-21	22.885944095392038	28.180635512017954	25.266727358487266	23.666693034102735
22-23	23.153547565353822	27.861259589201342	25.270119663665046	23.71507318177979
24-25	23.035330842620805	27.77510942863397	25.305107052068355	23.884452676676872
26-27	22.706192623981085	28.18821004873198	25.301469957804184	23.804127369482746
28-29	23.47811839963927	27.579606314301568	25.136083602230087	23.806191683829077
30-31	23.146174993195057	27.700161247842548	25.368293092643015	23.785370666319388
32-33	22.83121958922337	28.03250001897213	25.450002951427358	23.68627744037715
34-35	23.093468484488536	28.06271781185116	24.918990867004336	23.924822836655967
36-37	22.81653401026514	27.705002220168268	25.43631573849437	24.042148031072223
38-39	22.496293441868193	28.00223635972304	25.693709702580275	23.807760495828497
40-41	23.0629800348011	27.70051358243209	25.225103147633774	24.011403235133034
42-43	22.659340720184094	27.755822185101042	25.462164508810936	24.122672585903928
44-45	22.78199455768465	27.58814013812345	25.53969814308679	24.090167161105107
46-47	23.056269567006353	27.502384857196866	25.236858011255265	24.204487564541516
48-49	22.895251889210737	27.422990486090388	25.591052444541695	24.090705180157183
50-51	22.771440756893533	27.462453335426957	25.68533768999201	24.0807682176875
52-53	22.912345999694995	27.485346948937156	25.2177803525104	24.384526698857446
54-55	22.902373414642167	27.16977101385768	25.698359530303566	24.229496041196587
56-57	22.620223239612294	27.675320293470268	25.458638743887647	24.24581772302979
58-59	22.766332854957735	27.743997647692552	25.481106866976216	24.008562630373493
60-61	22.838866056343267	27.34976136973733	25.791441696482142	24.019930877437258
62-63	22.67400736438831	27.666796776876783	25.627200780360425	24.03199507837448
64-65	23.072148086804315	27.447793190416142	25.480988500122336	23.999070222657206
66-67	23.02107975255494	27.36805309205037	25.764642642956716	23.84622451243797
68-69	22.75568062494205	27.43172092923909	25.92632706350682	23.88627138231204
70-71	23.09223342469307	27.591023949372477	25.590624028494986	23.726118597439463
72-73	22.782171568966444	27.455419371384117	25.635021154491533	24.12738790515791
74-75	22.75287466051733	24.22364605690996	27.316505499390665	25.706973783182047
76	24.070102428642144	0.0	38.40714615810949	37.522751413248365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62306.0
1	36354.5
2	9603.0
3	8630.0
4	8457.5
5	7885.5
6	8196.5
7	10472.5
8	11869.5
9	12672.0
10	16055.0
11	37005.0
12	55386.5
13	68909.0
14	63195.0
15	36785.5
16	22746.0
17	16610.5
18	17571.0
19	20407.5
20	26789.5
21	33149.5
22	41277.0
23	56826.5
24	79592.0
25	99947.0
26	117587.0
27	148898.5
28	190676.5
29	218703.5
30	269030.0
31	353658.0
32	432339.0
33	484849.5
34	578692.5
35	697061.0
36	768501.0
37	804242.0
38	829162.5
39	895249.0
40	967653.0
41	1008234.5
42	1029446.0
43	1104558.0
44	1229203.5
45	1314043.5
46	1361738.0
47	1459184.0
48	1527752.5
49	1501384.0
50	1470904.0
51	1446841.0
52	1425278.5
53	1383367.0
54	1329080.5
55	1233244.0
56	1098916.0
57	952791.5
58	848472.5
59	755225.0
60	630194.0
61	520686.5
62	444283.0
63	375363.5
64	291528.0
65	224313.0
66	169365.5
67	144431.5
68	127481.0
69	99622.5
70	75842.0
71	63421.0
72	57144.5
73	53009.5
74	43214.0
75	32561.0
76	26304.5
77	19842.5
78	15761.0
79	13707.0
80	12717.0
81	11091.5
82	9784.5
83	9292.5
84	8405.0
85	7287.5
86	6688.0
87	6347.0
88	5272.0
89	3157.0
90	1527.5
91	904.5
92	781.0
93	760.5
94	714.5
95	666.5
96	644.0
97	618.0
98	552.5
99	426.5
100	341.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2404056421219782
2	0.2113060165318454
3	0.07469524463430197
4	0.07584922846471041
5	0.07733185493937757
6	0.07541975228187232
7	0.07493052289098717
8	0.07657000153677783
9	0.07688370587902477
10-11	0.07556726801423845
12-13	0.07482035291365045
14-15	0.07523115621897385
16-17	0.07567743799157517
18-19	0.07710404583369823
20-21	0.07587350320547952
22-23	0.07523302350672531
24-25	0.08145295900687181
26-27	0.07869124042244771
28-29	0.0825789335210082
30-31	0.09037299259564388
32-33	0.10096798329748452
34-35	0.11079178415796806
36-37	0.002832267827321921
38-39	0.004061048174109176
40-41	0.0011407368789966349
42-43	0.0021940770581186323
44-45	0.0014181850008488529
46-47	1.796605101314211E-4
48-49	0.004202791406064738
50-51	0.0040003026446195555
52-53	0.0015870104227424776
54-55	0.009947250700638146
56-57	0.004282539643176846
58-59	0.004140844733076381
60-61	0.0010425758875490798
62-63	3.628783154504204E-4
64-65	6.530984429963728E-4
66-67	0.00511032450051807
68-69	0.0037377776698520417
70-71	0.003839576739572016
72-73	0.001344341898500136
74-75	2.676297607456846E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	30724.0
36	1458.0
37	1732.0
38	2053.0
39	2423.0
40	2623.0
41	3081.0
42	3307.0
43	3409.0
44	3392.0
45	3559.0
46	3975.0
47	4211.0
48	4837.0
49	5234.0
50	5614.0
51	6311.0
52	6934.0
53	7367.0
54	7750.0
55	7912.0
56	8309.0
57	8840.0
58	9346.0
59	10149.0
60	10975.0
61	12017.0
62	12653.0
63	13750.0
64	14373.0
65	15389.0
66	17159.0
67	17473.0
68	17525.0
69	18891.0
70	21600.0
71	45573.0
72	144580.0
73	627204.0
74	1944317.0
75	6663460.0
76	1.7025316E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.117420332988335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62527873708818	22.020130287872025
2	11.07316460580166	6.448439764869921
3	4.132996603004581	3.6102659797349195
4	2.1108458830646697	2.4584954734140774
5	1.3312694869806752	1.9381566614449035
6	0.8899922225868835	1.5548566582891685
7	0.6422158765085227	1.3089768734572031
8	0.5037212002187117	1.1733649533924484
9	0.3812005824795457	0.998961983211323
>10	2.669958519401704	15.277004102995583
>50	0.3196084001100966	6.517818296216234
>100	0.26192355975266385	15.531602946018875
>500	0.03490242756135968	7.087214623447929
>1k	0.02198740524542984	11.486371405532653
>5k	7.719701613463198E-4	1.5228814032185047
>10k+	1.625200339676282E-4	1.0654585868841577
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72701	0.2715073736392374	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33965	0.12684485695735545	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32216	0.12031308440271346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.7345755029399513E-6	0.0
3	0.0	0.0	0.0	3.7345755029399513E-6	0.0
4	0.0	0.0	0.0	1.1203726508819854E-5	0.0
5	0.0	0.0	0.0	1.1203726508819854E-5	0.0
6	0.0	0.0	0.0	1.1203726508819854E-5	0.0
7	3.7345755029399513E-6	0.0	0.0	7.469151005879902E-5	0.0
8	3.7345755029399513E-6	0.0	0.0	7.469151005879902E-5	0.0
9	3.7345755029399513E-6	0.0	0.0	8.589523656761888E-5	0.0
10	1.4938302011759805E-5	3.7345755029399513E-6	0.0	8.589523656761888E-5	0.0
11	3.361117952645956E-5	3.7345755029399513E-6	0.0	8.589523656761888E-5	0.0
12	3.734575502939951E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
13	4.481490603527942E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
14	5.601863254409927E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
15	5.975320804703922E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
16	7.095693455585907E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
17	7.469151005879902E-5	3.7345755029399513E-6	0.0	8.962981207055883E-5	0.0
18	7.469151005879902E-5	3.7345755029399513E-6	0.0	9.336438757349878E-5	0.0
19	7.842608556173898E-5	3.7345755029399513E-6	0.0	9.336438757349878E-5	0.0
20	8.216066106467892E-5	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
21	8.962981207055883E-5	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
22	9.336438757349878E-5	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
23	9.709896307643873E-5	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
24	1.1203726508819854E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
25	1.1950641609407844E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
26	1.1950641609407844E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
27	1.2697556709995834E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
28	1.307101426028983E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
29	1.3444471810583825E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
30	1.456484446146581E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
31	1.6058674662641791E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
32	1.680558976322978E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
33	1.755250486381777E-4	3.7345755029399513E-6	0.0	1.0456811408231863E-4	0.0
34	1.904633506499375E-4	3.7345755029399513E-6	0.0	1.1203726508819854E-4	0.0
35	2.1287080366757722E-4	3.7345755029399513E-6	0.0	1.1577184059113848E-4	0.0
36	2.5021655869697675E-4	3.7345755029399513E-6	0.0	1.2697556709995834E-4	0.0
37	2.8009316272049634E-4	3.7345755029399513E-6	0.0	1.2697556709995834E-4	0.0
38	3.02500615738136E-4	3.7345755029399513E-6	0.0	1.2697556709995834E-4	0.0
39	3.2490806875577576E-4	3.7345755029399513E-6	0.0	1.2697556709995834E-4	3.7345755029399513E-6
40	3.361117952645956E-4	7.4691510058799025E-6	0.0	1.3444471810583825E-4	3.7345755029399513E-6
41	3.4731552177341544E-4	7.4691510058799025E-6	0.0	1.4191386911171814E-4	3.7345755029399513E-6
42	3.80926701299875E-4	7.4691510058799025E-6	0.0	1.53117595620538E-4	3.7345755029399513E-6
43	3.9586500331163484E-4	7.4691510058799025E-6	0.0	1.5685217112347796E-4	3.7345755029399513E-6
44	4.145378808263346E-4	7.4691510058799025E-6	0.0	1.6432132212935784E-4	3.7345755029399513E-6
45	4.4067990934691426E-4	7.4691510058799025E-6	0.0	1.680558976322978E-4	3.7345755029399513E-6
46	4.742910888733738E-4	7.4691510058799025E-6	0.0	1.829941996440576E-4	3.7345755029399513E-6
47	5.004331173939535E-4	7.4691510058799025E-6	0.0	1.904633506499375E-4	3.7345755029399513E-6
48	5.601863254409927E-4	7.4691510058799025E-6	0.0	1.9793250165581742E-4	3.7345755029399513E-6
49	5.863283539615723E-4	7.4691510058799025E-6	0.0	2.0166707715875738E-4	3.7345755029399513E-6
50	6.12470382482152E-4	7.4691510058799025E-6	0.0	2.0166707715875738E-4	3.7345755029399513E-6
51	6.311432599968518E-4	7.4691510058799025E-6	0.0	2.1287080366757722E-4	3.7345755029399513E-6
52	6.423469865056717E-4	7.4691510058799025E-6	0.0	2.2407453017639708E-4	3.7345755029399513E-6
53	6.759581660321311E-4	7.4691510058799025E-6	0.0	2.2407453017639708E-4	3.7345755029399513E-6
54	6.946310435468309E-4	7.4691510058799025E-6	0.0	2.27809105679337E-4	3.7345755029399513E-6
55	7.133039210615306E-4	7.4691510058799025E-6	0.0	2.4648198319403677E-4	3.7345755029399513E-6
56	7.431805250850503E-4	7.4691510058799025E-6	3.7345755029399513E-6	2.539511341999167E-4	3.7345755029399513E-6
57	7.879954311203298E-4	7.4691510058799025E-6	3.7345755029399513E-6	2.539511341999167E-4	3.7345755029399513E-6
58	8.514832146703089E-4	7.4691510058799025E-6	3.7345755029399513E-6	2.651548607087365E-4	3.7345755029399513E-6
59	9.485821777467476E-4	7.4691510058799025E-6	3.7345755029399513E-6	2.688894362116765E-4	3.7345755029399513E-6
60	0.0010083353857937869	7.4691510058799025E-6	3.7345755029399513E-6	2.688894362116765E-4	3.7345755029399513E-6
61	0.0010568848673320063	7.4691510058799025E-6	3.7345755029399513E-6	2.7262401171461643E-4	3.7345755029399513E-6
62	0.0010830268958525859	7.4691510058799025E-6	3.7345755029399513E-6	2.763585872175564E-4	3.7345755029399513E-6
63	0.0010830268958525859	7.4691510058799025E-6	3.7345755029399513E-6	2.8382773822343627E-4	3.7345755029399513E-6
64	0.0010830268958525859	7.4691510058799025E-6	3.7345755029399513E-6	2.912968892293162E-4	3.7345755029399513E-6
65	0.001086761471355526	1.1203726508819854E-5	7.4691510058799025E-6	2.9503146473225617E-4	7.4691510058799025E-6
>>END_MODULE
