FastQCFastQC Report
Tue 6 Oct 2015
G113-M1_TTAGGCAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M1_TTAGGCAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38837188
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1415440.36445481068299795TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG176650.039.58057843
CTCGTAT177100.038.89963542
CGTATGC179300.038.83622444
GATCGGA185750.037.493791
ATCGGAA195400.035.3490372
TCTCGTA193450.034.82813641
CGTCTGA206950.034.11844316
ATCTCGT199000.033.8804640
ACACGTC206550.033.8069713
ACGTCTG212700.033.18578315
CATCTCG208800.032.3644439
CACGTCT221700.031.77896314
CACACGT226600.030.93193812
TCGGAAG228000.030.7284893
TTAGGCA229250.030.62026434
CGGAAGA227400.030.5582984
CTTAGGC230550.030.4499633
ACTTAGG231650.030.20730432
GCACACG234300.029.81220611
TAGGCAT251450.027.3305135
Produced by FastQC (version 0.11.2)