FastQCFastQC Report
Tue 6 Oct 2015
G113-M2_TGACCAAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M2_TGACCAAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25234273
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36100.032.1485644
TCGTATG36550.031.9331643
GATCGGA39900.029.5956341
CTCGTAT39800.028.4993542
CGTCTGA47950.024.14546816
AATCTCG46750.024.12325339
ACACGTC49050.023.74963613
ATCGGAA50150.023.3571342
ACGTCTG51800.023.05827515
ATCTCGT49850.022.49054540
TCTCGTA53200.021.27995941
CACACGT59900.019.48431812
CACGTCT61650.019.18315714
GCACACG62750.018.5293211
GACCAAT64750.018.2665135
CGGAAGA68150.018.1231544
ACCAATC64800.017.6412536
TCGGAAG71200.017.5320073
ACTGACC81950.014.73239332
TATCGCG5250.014.24195432
Produced by FastQC (version 0.11.2)