FastQCFastQC Report
Tue 6 Oct 2015
G113-M2_TGACCAAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M2_TGACCAAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25234273
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT34450.032.50495511
CGTCGTG36800.030.42802812
TCGTGTA39600.028.22049314
GTCGCCG38750.027.76137544
AGCGTCG39400.027.75116310
CGTGTAG40800.027.44439715
GTCGTGT43250.026.2462613
GATCTCG45250.025.23115334
TCTCGGT45350.024.93347236
GGTCGCC43150.024.87966743
GTGGTCG43750.024.78968241
GATCGGA45300.024.7713411
ATCTCGG47600.024.12409635
GAGCGTC49150.022.783239
AGAGCGT51000.022.0430988
TGGTCGC49600.021.73284142
ATCGGAA53050.020.818762
TATCGCG3850.019.42703432
CTCGGTG59400.019.18427737
AAGAGCG59000.018.6813227
Produced by FastQC (version 0.11.2)