FastQCFastQC Report
Tue 6 Oct 2015
G113-M3_ACAGTGAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M3_ACAGTGAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24801362
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG304610.1228198677153295Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT47400.034.94930311
GTCGCCG48000.033.18173644
AGCGTCG50050.032.3515910
CGTCGTG52450.031.75087212
CGTGTAG54550.030.32612615
GGTCGCC53450.029.75745843
TCGTGTA56400.029.68242614
ATCTCGG57950.028.96361235
GATCTCG58150.028.7884534
TGGTCGC56950.028.08298742
GATCGGA59900.028.0120491
GTGGTCG58650.027.45653241
TCTCGGT61400.027.44465436
GAGCGTC60150.027.2850729
AGAGCGT62150.026.6549268
GTCGTGT64150.026.33721413
ATCGGAA63550.025.8262772
CGGTGGT73150.023.21697239
CTCGGTG73200.023.1710637
GTAGATC74100.022.44331231
Produced by FastQC (version 0.11.2)