FastQCFastQC Report
Tue 6 Oct 2015
G113-M4_GCCAATAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M4_GCCAATAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22218481
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23750.026.93160643
CGTATGC24100.026.72289344
GATCGGA25950.025.4391461
CTCGTAT26750.023.74877242
TATCTCG26050.023.71330539
CGCCAAT27300.023.67734133
GTCACGC28450.022.82096929
ACGCCAA28700.022.75677932
ATCGGAA30350.021.5787332
TCACGCC33350.019.8561830
AGTCACG33800.019.02110328
ACACGTC35200.018.60766213
CGTCTGA37550.018.11195816
TCTCGTA34600.018.04334841
ATCTCGT35600.018.0311940
CGCGATA6250.017.9376341
ACGTCTG38950.017.28083215
CACGCCA39650.016.8066731
TATCGCG6950.016.76977732
CACGTCT43150.015.23284214
Produced by FastQC (version 0.11.2)