FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext11_GGCTAC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext11_GGCTAC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15002469
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG712160.47469519850365965Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG87900.042.79241644
TGGTCGC87550.041.70682542
GATCTCG103000.041.4871834
GGTCGCC90600.040.9584543
GTGGTCG93350.040.67070441
ATCTCGG107300.040.25543235
GATCGGA158100.040.0953641
TCTCGGT105550.039.7577436
TCGGTGG111450.039.74764338
CGGTGGT101200.039.7092339
ATCGGAA159300.039.5768282
CGTCGTG156950.039.26789512
CGTGTAG158100.039.23119415
CTCGGTG107800.039.19432437
GCGTCGT158000.039.05173511
AGCGTCG158150.038.87602610
TCGGAAG163350.038.828893
GTCGTGT161600.038.2420813
GAGCGTC160600.038.1458939
TCGTGTA160050.038.1115514
Produced by FastQC (version 0.11.2)