FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext12_CTTGTA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext12_CTTGTA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18023263
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2574881.4286425271605925TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC319300.042.3729244
TCGTATG322050.042.0255743
CTCGTAT309400.041.9315242
TCTCGTA288800.041.8785141
CGTCTGA332000.041.7485216
AATCTCG288700.041.71021339
ACGTCTG335200.041.47471215
ATCTCGT291300.041.4360640
GATCGGA337450.040.8382641
ACACGTC346200.040.20141213
ATCGGAA344550.039.9990272
TCGGAAG346800.039.72643
CGGAAGA350050.039.238224
CACACGT356550.039.05369612
GCACACG356550.039.00855311
CACGTCT357600.038.98752214
GTAATCT312150.038.5063237
GAACTCC361250.038.1455921
AGTCACC358300.038.08843228
CACGACG17000.038.0605415
Produced by FastQC (version 0.11.2)