FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext12_CTTGTA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext12_CTTGTA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18023263
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1461120.810685612255672Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN199020.11042395597290014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG178100.043.14113244
TGGTCGC176500.042.29852742
GGTCGCC181650.042.10450743
GTGGTCG186050.042.08971841
GATCTCG207250.041.9260234
TCGGTGG217700.041.52400238
ATCTCGG213850.041.36234735
CGGTGGT202250.041.25979239
TCTCGGT206450.041.2587336
CTCGGTG208400.040.90603637
CGTGTAG307450.040.67163515
GATCGGA316350.040.2857631
ATCGGAA316800.040.1634452
CGTCGTG310250.040.16284612
GCGTCGT309750.040.07568711
TCGGAAG319950.040.008923
AGCGTCG313250.039.9583210
GTCGTGT313500.039.78451513
TCGTGTA311550.039.6819714
GAGCGTC317150.039.404159
Produced by FastQC (version 0.11.2)