FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext13_AGTCAA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext13_AGTCAA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18689009
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1899581.0164155841543014TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT229800.042.226344
TCTCGTA213000.041.6870343
CGTCTGA252700.041.5860216
ACGTCTG255250.041.18781315
AATCTCG214800.041.13471641
ATCTCGT215350.041.07895742
GATCGGA256400.040.392691
ACACGTC264050.039.85660613
ATCGGAA261000.039.577292
TCGGAAG264850.039.1094783
CGGAAGA264950.038.8789524
GCACACG274900.038.34631311
CACACGT275850.038.21752512
CACGTCT276400.038.12372614
AGTCAAC267750.038.1229434
GTCAACA273900.037.2687435
GAACTCC283100.037.00003421
ACGACGC15450.036.3237716
CAGTCAA281550.036.28570633
CGACGCT15700.036.165917
Produced by FastQC (version 0.11.2)