FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext13_AGTCAA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext13_AGTCAA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18689009
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1172020.6271172537826911Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193090.1033174097139126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG140700.042.78988344
TGGTCGC138700.042.3445542
GTGGTCG146600.041.83290541
GATCTCG162200.041.63961434
GGTCGCC143950.041.62548443
TCGGTGG171000.041.32703838
CGGTGGT157900.040.92494639
TCTCGGT163350.040.63464436
CGTGTAG236400.040.52429215
CTCGGTG163700.040.45523537
ATCTCGG170250.040.27821435
CGTCGTG240050.039.98750312
GATCGGA242650.039.9528731
AGCGTCG240700.039.8736910
GCGTCGT240700.039.7919211
ATCGGAA244350.039.6259882
GTCGTGT242650.039.48254813
TCGGAAG247100.039.3545343
TCGTGTA241100.039.30016714
GAGCGTC244350.039.2238129
Produced by FastQC (version 0.11.2)