FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext14_AGTTCC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext14_AGTTCC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17550119
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1788481.0190700131435007Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182430.10394801311603642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG214350.043.28434844
TGGTCGC211650.042.52691342
GTGGTCG222650.042.34232741
GATCTCG237750.042.3378534
TCGGTGG248800.042.1854938
GGTCGCC219800.042.17106643
ATCTCGG244250.041.8144235
TCTCGGT236150.041.760436
CGGTGGT234300.041.57635539
CTCGGTG239400.041.43387237
GATCGGA362000.040.275131
CGTGTAG356600.040.22179815
ATCGGAA363400.040.0386282
TCGGAAG365950.039.745693
CGTCGTG364900.039.2228912
GCGTCGT364850.039.1650311
AGCGTCG366600.039.12875710
GTCGTGT366800.038.8933613
TCGTGTA364700.038.79497514
GAGCGTC368250.038.702139
Produced by FastQC (version 0.11.2)