FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext15_ATGTCA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext15_ATGTCA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259458
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2492811.1198880044608455TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT312300.041.95686344
TCTCGTA286500.041.56129543
CGTCTGA340200.041.46038416
AATCTCG288550.041.06908441
ACGTCTG346350.040.92752515
ATCTCGT291200.040.76212342
GATCGGA343600.040.72551
ATCGGAA351000.039.877682
ACACGTC357350.039.78443513
TCGGAAG354600.039.3238453
CGGAAGA357950.038.76544
GCACACG369250.038.30912811
CACACGT371700.038.2889612
CACGTCT372950.038.2148914
GAACTCC375900.037.31002421
CGACGCT21350.036.4907517
GTCACAT381550.036.3484329
CACGACG21450.036.3206315
ACGACGC21450.036.3206316
ACATGTC379400.036.27057632
Produced by FastQC (version 0.11.2)