FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext15_ATGTCA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext15_ATGTCA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259458
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1577570.7087189634177076Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN255690.11486802598697596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG191200.043.14074744
TGGTCGC188000.042.52984242
GGTCGCC193550.042.29893543
GTGGTCG197400.042.09805341
GATCTCG217600.041.9208734
TCGGTGG229750.041.24165738
TCTCGGT218550.041.15734536
ATCTCGG224800.041.12668635
CGGTGGT212300.041.11942339
CTCGGTG221300.040.68748537
CGTGTAG321250.040.2039115
GATCGGA325200.040.1716041
ATCGGAA324650.040.0133632
CGTCGTG325600.039.54882412
GCGTCGT324750.039.5275611
TCGGAAG330500.039.438913
AGCGTCG325650.039.41138510
GTCGTGT329200.039.19946313
TCGTGTA327400.039.0244914
GAGCGTC328150.038.9497689
Produced by FastQC (version 0.11.2)