FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext16_CCGTCC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext16_CCGTCC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15505260
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17200.032.6002444
ACCCGTC17400.031.59446732
TCGTATG18000.031.27451143
CTCGTAT17650.030.5242142
TCTCGTA16250.030.17767541
CGTCTGA20650.029.30685416
CACCCGT18850.029.28087431
CCGTCCA19100.028.89780234
CGTCCAT18050.027.7775135
CACGACG5750.027.55631815
TCACCCG20150.027.50082830
CGACGCT5550.027.35978317
ACACGTC22800.026.83283213
ACGTCTG22950.026.46567315
ACGACGC5800.026.18048116
GATCGGA23100.025.2161141
ATCGGAA24200.024.62292
CATCTCG20550.023.86312539
ATCTCGT21200.023.3389340
CCCGTCC24200.023.2619633
Produced by FastQC (version 0.11.2)