FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext16_CCGTCC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext16_CCGTCC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15505260
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG14450.033.66287612
GTCGCCG9550.032.2468844
GCGTCGT15300.032.08323311
AGCGTCG16100.031.71943310
CGTGTAG18600.027.08947415
GATCTCG14300.026.76019334
GTCGTGT18550.026.3384113
TGGTCGC11100.026.1588242
GAGCGTC20050.025.9094459
TCGTGTA19050.025.5281214
GATCGGA21850.023.9669061
GGTCGCC13100.023.84435843
ATCGGAA22450.023.4271762
ATCTCGG16350.023.4050935
GTGGTCG13550.022.72757541
AGAGCGT23350.022.2477258
AAGAGCG23250.021.9643577
TCGGTGG17100.021.48055538
TCGGAAG25350.021.2776183
TCTCGGT16850.021.14494536
Produced by FastQC (version 0.11.2)