FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext18_GTCCGC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext18_GTCCGC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16643386
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC18800.040.23660733
ACGTCCG19800.038.64869732
GTCCGCA22150.034.54795534
TCCGCAC22600.033.56926735
TCTCGTA19450.032.7862243
CTCGTAT22200.032.78458444
CCGCACA24450.031.02926336
CGTCTGA25800.029.76965116
ACACGTC26900.028.96127713
CACGACG6250.028.8736615
CGCACAT23800.027.62638937
ACGTCTG28250.027.34366215
GTCACGT28150.027.1842729
AGTCACG29000.026.61592928
ATCTCGT24200.026.35090642
GATCGGA29650.026.096461
CGACGCT7000.025.78005217
ACGACGC7200.025.67525716
ATCGGAA30150.025.451262
CATCTCG25300.025.11928241
Produced by FastQC (version 0.11.2)