FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext18_GTCCGC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext18_GTCCGC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16643386
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG19350.034.46859712
GCGTCGT19650.034.2814211
AGCGTCG20350.033.8593410
GTCGCCG12050.032.85742644
GTCGTGT23550.028.50475313
TCGTGTA22800.028.4733114
CGTGTAG24200.028.00505415
GAGCGTC24500.027.9437739
GATCTCG16950.026.85730734
TGGTCGC14200.025.71381842
GTGGTCG15400.025.56688541
AGAGCGT27400.025.3882338
GGTCGCC15950.024.82324643
GATCGGA28450.024.0528981
TCGGTGG20450.023.66142538
AAGAGCG29350.023.3255887
ATCGGAA29400.023.0532382
ATCTCGG20700.021.99198735
TCGGAAG31900.021.6719383
TCTCGGT21000.021.57367536
Produced by FastQC (version 0.11.2)