FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M1M19-NEBNext19_GTGAAA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext19_GTGAAA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24855199
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17550.022.30189544
TCTCGTA16200.021.58203343
AACGATC16400.020.5149338
GAAACGA24250.017.31981536
CGATCTC19250.017.24919140
TGAAACG24150.017.20942135
CGTCTGA28200.017.01339116
GATCGGA26650.016.6045671
ATCGGAA27150.016.5210722
ACACGTC28250.016.51571513
ATCTCGT22000.016.09212542
AAACGAT23100.015.89743137
GATCTCG21800.015.4332541
ACGATCT21900.014.96113939
ACGTCTG31950.014.46544815
AGTCACG30150.013.85762328
GTCACGT30850.013.685389529
CGGAAGA40250.011.7994394
TCGGAAG39550.011.730313
CACGTCT40350.011.5630714
Produced by FastQC (version 0.11.2)