FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M1M19-NEBNext19_GTGAAA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext19_GTGAAA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24855199
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN261900.10537030904479985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG15750.025.57558612
GCGTCGT16150.025.2172211
AGCGTCG16950.024.28697610
GTCGCCG12200.020.0130644
GTCGTGT22350.018.8089113
TCGTGTA21450.018.0562114
CGTGTAG23550.017.09820715
GAGCGTC24350.017.0867379
GATCGGA26600.015.5518631
ATCGGAA26950.015.5147822
TGGTCGC16200.015.20725142
GATCTCG22100.014.62786234
GTGGTCG19150.013.55360541
AGAGCGT31300.013.1521058
GGTCGCC21900.012.15320143
AAGAGCG34400.011.96657
ATCTCGG27000.011.40313935
TCTCGGT25950.011.18683536
TCGGAAG38350.011.0806973
TCGGTGG29000.010.16284138
Produced by FastQC (version 0.11.2)