FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext5_ACAGTG-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext5_ACAGTG-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14990255
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC360850.24072305641231587TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49500.040.02447544
TCTCGTA44150.039.74672741
CTCGTAT48100.039.4992842
TCGTATG50600.039.1991443
GATCGGA51300.038.881721
GATCTCG45450.038.41632539
CGTCTGA52350.038.25542416
ATCGGAA52500.037.9862562
ATCTCGT46900.037.1817340
ACACGTC55000.036.53212713
ACGTCTG55000.036.37219615
TCGGAAG55000.036.29913
CGGAAGA56400.035.476034
GCACACG61150.032.94419511
CACACGT63500.031.63228412
CACGTCT70050.028.65200614
AGTGATC63700.027.61720736
GAACTCC78350.025.92421
GTCACAC77600.025.87154229
GTGATCT70100.025.22128537
Produced by FastQC (version 0.11.2)