FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext5_ACAGTG-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext5_ACAGTG-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14990255
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG215280.14361330077440312Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154200.10286682915000445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG43850.041.0653210
CGTCGTG44200.040.48610712
GCGTCGT44650.040.18123611
GTCGCCG27500.040.07371544
GAGCGTC46700.038.3233689
GTCGTGT47250.038.10079613
GATCGGA48900.038.0678521
TCGTGTA47950.037.30959714
ATCGGAA49850.037.1688082
CGTGTAG48550.037.1616815
TGGTCGC28950.036.69878442
GATCTCG34700.036.3162834
GGTCGCC30400.036.17841743
AGAGCGT49800.036.1147168
GTGGTCG31450.035.3202641
TCGGAAG52300.035.2771383
AAGAGCG51800.035.27227
TCGGTGG37200.035.00198438
TCTCGGT36350.033.76095236
ATCTCGG37900.033.42419435
Produced by FastQC (version 0.11.2)