FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext7_CAGATC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext7_CAGATC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15532272
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC252660.1626677668276734TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32900.037.96143744
CTCGTAT32700.037.05169742
GATCGGA33850.036.588121
TCGTATG34150.036.57310543
TCTCGTA31250.036.16903341
CGTCTGA35900.035.98440616
ATCGGAA36300.034.586542
ACGTCTG37650.034.4287315
ATCTCGT33550.033.82068340
CATCTCG33900.033.21203239
ACACGTC39700.033.0938713
TCGGAAG39300.032.225883
CGGAAGA41350.030.3091814
CACACGT46400.028.02324912
GCACACG45950.027.95270711
CACGTCT52050.025.15730514
GATCATC48200.023.54123536
CGACGCT3900.021.44320517
GAACTCC61850.021.17002521
AGTCACC62100.020.99836528
Produced by FastQC (version 0.11.2)