FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext7_CAGATC-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext7_CAGATC-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15532272
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN164250.10574756867507858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG29250.039.2828112
AGCGTCG29200.039.05219710
GCGTCGT29550.038.8130211
GTCGCCG18200.037.949944
GAGCGTC31650.035.8204239
CGTGTAG33600.034.90498415
GATCGGA34000.034.751861
TCGTGTA33650.034.39960514
ATCGGAA35200.033.759022
GTCGTGT34400.033.6551513
GATCTCG23350.032.77477634
GTGGTCG21150.032.44885641
TGGTCGC20150.032.42186742
AGAGCGT35100.032.3625348
TCGGAAG36700.031.9165273
GGTCGCC21400.031.86420643
AAGAGCG36300.031.7162957
CGGAAGA38350.030.2609564
TCTCGGT25350.029.32319636
CGGTGGT25500.029.23940539
Produced by FastQC (version 0.11.2)