FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext8_ACTTGA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext8_ACTTGA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13033918
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC367980.282324930999259TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC48350.040.29356444
TCGTATG48950.039.8910443
CTCGTAT47950.039.0261842
GATCGGA51450.038.595211
TCTCGTA44750.038.2797941
CGTCTGA53000.038.035716
ACGTCTG54050.037.5003915
AATCTCG46350.036.8160439
ATCGGAA54650.036.5729942
ACACGTC55450.036.4740613
ATCTCGT47500.036.06358740
TCGGAAG57150.034.8574373
CGGAAGA58000.034.1191334
GCACACG62200.032.49427811
CACACGT62450.032.44583512
CACGTCT67300.030.2807114
CGACGCT4250.028.99819217
GAACTCC72400.027.9027921
GTCACAC71650.027.71309529
CCAGTCA77400.026.0851626
Produced by FastQC (version 0.11.2)