FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext8_ACTTGA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext8_ACTTGA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13033918
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135700.1041129766199235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG26750.040.21047644
GCGTCGT44300.039.554811
AGCGTCG44550.039.53124210
CGTCGTG44700.039.29525412
GTGGTCG29000.037.5456641
CGTGTAG47100.037.51290515
GATCGGA47950.037.3981441
TGGTCGC27700.037.32291842
TCGTGTA47150.037.10464514
GAGCGTC47500.036.9831739
GTCGTGT47450.036.96778513
GATCTCG31550.036.8735834
GGTCGCC28900.036.838943
ATCGGAA48700.036.7350852
AGAGCGT49750.035.532228
TCGGAAG51200.035.13183
AAGAGCG50400.034.9421237
TCGGTGG35950.034.87292538
TCTCGGT33250.034.79123336
CGGTGGT33700.034.00323539
Produced by FastQC (version 0.11.2)