FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext9_GATCAG-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext9_GATCAG-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258956
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC340630.20950299637934933TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46300.039.55980344
CTCGTAT44700.038.51817742
TCGTATG48200.038.3672343
CGATCAG47750.038.3144733
ACGATCA48300.038.151132
GTCACGA48400.038.0725129
CGTCTGA49750.037.6003116
GATCGGA50100.037.595371
TCTCGTA41550.037.20624541
CACGATC50250.036.62673631
GATCTCG42700.036.25570339
ATCGGAA51850.036.0449372
ACGTCTG52500.035.75652715
ACACGTC52800.035.71975713
ATCTCGT44250.035.0851240
TCACGAT54250.034.04784830
TCGGAAG54400.034.031883
AGTCACG54700.033.97010828
CGGAAGA55350.033.2491534
GCACACG60550.031.20134711
Produced by FastQC (version 0.11.2)