Basic Statistics
Measure | Value |
---|---|
Filename | G124-G125-M20M38-NEBNext11_GGCTAC-_AC8KUAANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21388905 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC | 834912 | 3.9034817350397324 | TruSeq Adapter, Index 11 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 98425 | 0.0 | 43.402206 | 1 |
ATCGGAA | 98580 | 0.0 | 43.24215 | 2 |
CGTCTGA | 98815 | 0.0 | 43.137024 | 16 |
ACGTCTG | 99005 | 0.0 | 43.003094 | 15 |
TCGGAAG | 99270 | 0.0 | 42.99254 | 3 |
ACACGTC | 99350 | 0.0 | 42.96417 | 13 |
CGTATGC | 97790 | 0.0 | 42.92354 | 44 |
ACGGCTA | 97095 | 0.0 | 42.8895 | 32 |
CGGCTAC | 96740 | 0.0 | 42.864407 | 33 |
TCTCGTA | 91385 | 0.0 | 42.85104 | 41 |
TCGTATG | 97885 | 0.0 | 42.845932 | 43 |
CTCGTAT | 96555 | 0.0 | 42.83064 | 42 |
CGGAAGA | 99480 | 0.0 | 42.813454 | 4 |
AGTCACG | 98610 | 0.0 | 42.62947 | 28 |
CACGTCT | 100100 | 0.0 | 42.611862 | 14 |
ATCTCGT | 91790 | 0.0 | 42.605263 | 40 |
GTCACGG | 98075 | 0.0 | 42.590206 | 29 |
TCACGGC | 97925 | 0.0 | 42.583473 | 30 |
CATCTCG | 91390 | 0.0 | 42.577553 | 39 |
CACGGCT | 98025 | 0.0 | 42.444447 | 31 |