FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext11_GGCTAC-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext11_GGCTAC-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21388905
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC8349123.9034817350397324TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA984250.043.4022061
ATCGGAA985800.043.242152
CGTCTGA988150.043.13702416
ACGTCTG990050.043.00309415
TCGGAAG992700.042.992543
ACACGTC993500.042.9641713
CGTATGC977900.042.9235444
ACGGCTA970950.042.889532
CGGCTAC967400.042.86440733
TCTCGTA913850.042.8510441
TCGTATG978850.042.84593243
CTCGTAT965550.042.8306442
CGGAAGA994800.042.8134544
AGTCACG986100.042.6294728
CACGTCT1001000.042.61186214
ATCTCGT917900.042.60526340
GTCACGG980750.042.59020629
TCACGGC979250.042.58347330
CATCTCG913900.042.57755339
CACGGCT980250.042.44444731