FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext11_GGCTAC-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext11_GGCTAC-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21388905
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4876012.2796912698429397Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297060.13888509019045156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG918650.043.36633312
AGCGTCG914150.043.3406510
GCGTCGT918450.043.33950411
GATCGGA921950.043.273661
GTCGCCG583150.043.27137844
TCGTGTA915050.043.2091914
ATCGGAA919050.043.2033772
GTCGTGT923750.043.1669213
GATCTCG672050.043.14797234
GAGCGTC920350.043.1080679
TGGTCGC574100.043.0693642
CGTGTAG923800.043.06355315
GTGGTCG603350.042.9864241
AGAGCGT927600.042.9158948
TCGGAAG933500.042.9146423
ATCTCGG684100.042.8702735
GGTCGCC590450.042.75491343
TCGGTGG702600.042.7368538
TCTCGGT668500.042.72780636
AAGAGCG926950.042.7270747
Produced by FastQC (version 0.11.2)