FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext12_CTTGTA-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext12_CTTGTA-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20691334
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5907072.854852181111184TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA709150.043.327761
CGTCTGA714150.043.0878616
ATCGGAA713100.043.037492
ACACGTC716000.042.98851413
ACGTCTG715100.042.98751415
CGTATGC705400.042.9092444
TCGGAAG717950.042.8172073
CTCGTAT689300.042.8015742
TCTCGTA656100.042.77947641
TCGTATG707800.042.7668543
CGGAAGA718300.042.70464
AATCTCG654200.042.69937539
ATCTCGT659950.042.4773140
CACGTCT725500.042.43198414
GCACACG728150.042.25546611
CACACGT729850.042.21407712
GTAATCT676200.041.15078737
GAACTCC756600.040.56536521
AGTCACC745450.040.5404528
TGTAATC687850.040.4058636
Produced by FastQC (version 0.11.2)