Basic Statistics
Measure | Value |
---|---|
Filename | G124-G125-M20M38-NEBNext12_CTTGTA-_AC8KUAANXX_L008_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20691334 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 368297 | 1.7799577349628595 | Illumina Single End PCR Primer 1 (100% over 50bp) |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 32301 | 0.1561088328089431 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCGTG | 66920 | 0.0 | 43.512505 | 12 |
AGCGTCG | 66835 | 0.0 | 43.505577 | 10 |
GCGTCGT | 66890 | 0.0 | 43.459488 | 11 |
GTCGCCG | 44060 | 0.0 | 43.30239 | 44 |
GTCGTGT | 67515 | 0.0 | 43.239273 | 13 |
GATCGGA | 67850 | 0.0 | 43.23547 | 1 |
GAGCGTC | 67315 | 0.0 | 43.22089 | 9 |
ATCGGAA | 67645 | 0.0 | 43.16608 | 2 |
TCGTGTA | 67115 | 0.0 | 43.1604 | 14 |
TGGTCGC | 43235 | 0.0 | 43.06083 | 42 |
CGTGTAG | 67660 | 0.0 | 43.05851 | 15 |
GATCTCG | 50800 | 0.0 | 43.05445 | 34 |
AGAGCGT | 67990 | 0.0 | 42.95388 | 8 |
TCGGAAG | 68325 | 0.0 | 42.904377 | 3 |
GTGGTCG | 45325 | 0.0 | 42.87557 | 41 |
GGTCGCC | 44620 | 0.0 | 42.773613 | 43 |
AAGAGCG | 68145 | 0.0 | 42.772285 | 7 |
ATCTCGG | 51575 | 0.0 | 42.753094 | 35 |
TCTCGGT | 50115 | 0.0 | 42.705265 | 36 |
TCGGTGG | 52970 | 0.0 | 42.663883 | 38 |