FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext12_CTTGTA-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext12_CTTGTA-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20691334
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3682971.7799577349628595Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN323010.1561088328089431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG669200.043.51250512
AGCGTCG668350.043.50557710
GCGTCGT668900.043.45948811
GTCGCCG440600.043.3023944
GTCGTGT675150.043.23927313
GATCGGA678500.043.235471
GAGCGTC673150.043.220899
ATCGGAA676450.043.166082
TCGTGTA671150.043.160414
TGGTCGC432350.043.0608342
CGTGTAG676600.043.0585115
GATCTCG508000.043.0544534
AGAGCGT679900.042.953888
TCGGAAG683250.042.9043773
GTGGTCG453250.042.8755741
GGTCGCC446200.042.77361343
AAGAGCG681450.042.7722857
ATCTCGG515750.042.75309435
TCTCGGT501150.042.70526536
TCGGTGG529700.042.66388338