FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M20M38-NEBNext13_AGTCAA-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext13_AGTCAA-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389894
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA10300.030.99121516
TCTCGTA7950.030.98328243
ACGTCTG10700.030.86137815
CTCGTAT8350.030.81597144
ACACGTC10800.030.16795213
ATCTCGT8500.029.23775942
AATCTCG8450.029.15211541
CACGTCT12700.026.52134314
GCACACG13950.023.96859611
GATCGGA13700.022.822561
ATCGGAA14050.022.5394082
TCGGAAG14200.022.4561863
CACACGT15100.021.8607412
CGGAAGA14900.020.958614
GAACTCC22200.015.36702321
AGTCAAC18650.015.21344934
CACGACG900.0043426214.676315
GTCAACA19800.014.21875635
TCTGAAC24250.013.61718618
CAATCTC19000.013.31254540
Produced by FastQC (version 0.11.2)