FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M20M38-NEBNext13_AGTCAA-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext13_AGTCAA-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389894
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86440.1603742114408929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG4800.036.66053844
CGTCGTG8200.035.43507412
GCGTCGT8200.035.16695411
AGCGTCG8500.033.92387410
GTGGTCG5800.032.23658841
TGGTCGC5500.031.99524742
GAGCGTC9350.031.3098499
TCGTGTA9500.030.5783814
CGTGTAG9650.030.55718615
GTCGTGT9600.030.49278813
GGTCGCC6050.029.81347543
AGAGCGT10450.027.804488
GATCTCG7650.027.02338634
TCTCGGT7600.026.62217736
ATCTCGG8250.025.591235
AAGAGCG11450.024.9944467
TCGGTGG8400.024.87332238
CGGTGGT7850.024.6571539
CTCGGTG8150.024.556237
ATCGGAA13100.021.5015872
Produced by FastQC (version 0.11.2)