FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M20M38-NEBNext14_AGTTCC-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext14_AGTTCC-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20475486
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT292800.1430002687115705TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC36100.036.92030738
CTCGTAT39200.035.9066344
TCCGTAT38350.035.27033637
TCTCGTA37850.034.86319743
AGTTCCG42150.034.64771734
CGTATCT38250.034.55754539
TATCTCG39950.033.30665641
CGTCTGA48800.032.48347516
TTCCGTA44900.032.32938436
GTTCCGT44900.032.2314235
ACACGTC51150.031.03404613
ACGTCTG51300.031.02921115
ATCTCGT43800.030.4789242
CACGTCT62350.025.91841114
CACACGT66950.024.22453712
GCACACG67300.023.9261211
CGGAAGA78950.019.9160844
GTATCTC69150.019.4652140
TCGGAAG80700.019.3751013
ATCGGAA81950.019.0258982
Produced by FastQC (version 0.11.2)