FastQCFastQC Report
Tue 15 Mar 2016
G124-G125-M20M38-NEBNext14_AGTTCC-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext14_AGTTCC-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20475486
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327270.15983503395230764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG24550.039.4216744
CGTCGTG40600.035.8364612
GCGTCGT43050.033.7994711
GATCTCG32950.033.7073934
GGTCGCC29350.033.3491443
TGGTCGC29300.033.03102542
AGCGTCG44600.032.87080410
TCGTGTA45000.032.47171414
CGTGTAG45550.032.31771515
GTCGTGT45350.032.27374313
GTGGTCG33100.030.966741
TCGGTGG36600.030.76815238
ATCTCGG37100.030.05566835
GAGCGTC51050.028.7603119
TCTCGGT37800.028.16147836
CGGTGGT36650.027.96699139
CTCGGTG39200.027.3243737
AGAGCGT58350.025.3890348
AAGAGCG61550.024.1062937
GTAGATC59300.019.84159331
Produced by FastQC (version 0.11.2)