FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext22_CGTACG-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext22_CGTACG-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14357716
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT730370.5086951155740927TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG93150.041.83817733
CGTACGT93250.041.8177734
ACCGTAC93950.041.482532
CGTAATC84350.041.14540538
GATCGGA99650.041.0744971
GTACGTA95850.040.70623435
CTCGTAT92650.040.3765944
CACCGTA97200.040.27649331
ATCGGAA101850.040.181612
TCTCGTA86750.039.9790943
TACGTAA97050.039.9762936
CGTCTGA103750.039.7486916
ACGTAAT90750.039.26158537
AATCTCG88400.039.13513641
GTCACCG100950.039.1064629
ACGTCTG105200.039.0543215
TCACCGT100650.038.9388230
ACACGTC105350.038.93601213
ATCTCGT90150.038.52047742
TCGGAAG106700.038.4582183
Produced by FastQC (version 0.11.2)