FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext22_CGTACG-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext22_CGTACG-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14357716
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG485670.33826410830246256Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN207970.14484894394066578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG91650.041.7191210
CGTCGTG91800.041.53135312
GTCGCCG64400.041.42959244
GCGTCGT92800.041.15911
GATCGGA95350.040.590261
TCGTGTA93950.040.33563214
GAGCGTC94900.040.3135769
GATCTCG74300.040.28579734
TGGTCGC64750.040.2547742
ATCGGAA95950.040.1571962
GTCGTGT95850.040.04677213
CGTGTAG95200.039.96364215
GTGGTCG68250.039.64055341
AGAGCGT97300.039.3185168
TCGGAAG98550.039.0948033
GGTCGCC68300.038.9671743
ATCTCGG77250.038.91847235
AAGAGCG98950.038.509857
TCGGTGG80000.038.1595638
TCTCGGT76900.038.0380836
Produced by FastQC (version 0.11.2)