FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext9_GATCAG-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext9_GATCAG-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23662176
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3598961.5209759237696483TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA430450.043.1778831
CGTATGC429100.042.8677344
ATCGGAA434450.042.802392
TCGTATG429650.042.7617643
CTCGTAT418250.042.749942
CGATCAG424700.042.745333
CGTCTGA436150.042.71708316
GTCACGA430200.042.70907229
ACGATCA426700.042.6378232
TCTCGTA392000.042.59547441
CACGATC427850.042.58472431
ACACGTC439550.042.4363913
GATCTCG391750.042.38776439
TCACGAT432700.042.35521730
ACGTCTG440250.042.3442715
TCGGAAG441100.042.162093
AGTCACG438850.042.04031428
ATCTCGT397500.042.00145740
CGGAAGA444150.041.8182754
CACGTCT450150.041.4765914