Basic Statistics
Measure | Value |
---|---|
Filename | G124-G125-M20M38-NEBNext9_GATCAG-_AC8KUAANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23662176 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC | 359896 | 1.5209759237696483 | TruSeq Adapter, Index 9 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 43045 | 0.0 | 43.177883 | 1 |
CGTATGC | 42910 | 0.0 | 42.86773 | 44 |
ATCGGAA | 43445 | 0.0 | 42.80239 | 2 |
TCGTATG | 42965 | 0.0 | 42.76176 | 43 |
CTCGTAT | 41825 | 0.0 | 42.7499 | 42 |
CGATCAG | 42470 | 0.0 | 42.7453 | 33 |
CGTCTGA | 43615 | 0.0 | 42.717083 | 16 |
GTCACGA | 43020 | 0.0 | 42.709072 | 29 |
ACGATCA | 42670 | 0.0 | 42.63782 | 32 |
TCTCGTA | 39200 | 0.0 | 42.595474 | 41 |
CACGATC | 42785 | 0.0 | 42.584724 | 31 |
ACACGTC | 43955 | 0.0 | 42.43639 | 13 |
GATCTCG | 39175 | 0.0 | 42.387764 | 39 |
TCACGAT | 43270 | 0.0 | 42.355217 | 30 |
ACGTCTG | 44025 | 0.0 | 42.34427 | 15 |
TCGGAAG | 44110 | 0.0 | 42.16209 | 3 |
AGTCACG | 43885 | 0.0 | 42.040314 | 28 |
ATCTCGT | 39750 | 0.0 | 42.001457 | 40 |
CGGAAGA | 44415 | 0.0 | 41.818275 | 4 |
CACGTCT | 45015 | 0.0 | 41.47659 | 14 |