FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M20M38-NEBNext9_GATCAG-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext9_GATCAG-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23662176
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2271420.959937074257245Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN362140.15304594133692523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG409600.043.22322512
AGCGTCG409200.043.17622410
GTCGCCG267800.043.15436644
GCGTCGT410050.043.1522411
GATCGGA414100.043.0041961
ATCGGAA414650.042.734862
TGGTCGC264700.042.63855742
GAGCGTC413950.042.637169
GTCGTGT416300.042.5961913
TCGTGTA414050.042.48197614
GATCTCG312300.042.3875934
GTGGTCG278450.042.3578641
CGTGTAG416100.042.3528115
AGAGCGT419150.042.198468
TCGGAAG421950.042.121063
GGTCGCC275600.041.92521343
AAGAGCG420550.041.871687
CGGAAGA422400.041.696074
TCTCGGT312350.041.6079536
ATCTCGG321250.041.5972535
Produced by FastQC (version 0.11.2)